Biology
06102026–2028 syllabus

BIOLOGY · CHAPTER 3

Movement into and out of cells

How concentration gradients, membranes and energy control particle movement.

Core + Supplement3 connected sectionsNotes only

LEARNING OBJECTIVES

By the end of this chapter, you should be able to:

  • explain diffusion and its rate
  • plan diffusion and osmosis investigations
  • describe osmosis using water potential
  • predict effects on plant cells
  • explain active transport and carrier proteins

THE BIG IDEA

How concentration gradients, membranes and energy control particle movement.

This chapter builds each process from its structures, inputs and outputs. Follow the connections rather than memorising isolated facts, then use precise biological terms when explaining cause and effect.

01

SECTION 01

Diffusion

Particles move randomly. When more particles begin in one region, random movement produces a net movement down the concentration gradient until distribution is more even.

DETAILED EXPLANATION

  • Diffusion supplies oxygen to cells and removes carbon dioxide; dissolved substances also cross membranes by diffusion.
  • A steeper gradient, higher temperature, larger surface area and shorter distance increase the rate.
  • Energy comes from particles' kinetic energy, not directly from respiration.
  • A diffusion investigation can compare how far a coloured substance travels through agar in a fixed time. Change one factor, such as temperature, while keeping agar size, concentration and time constant.
LABELLED STUDY DIAGRAMDown a concentration gradient
1high concentration
2net random movement
3low concentration
02

SECTION 02

Osmosis and water potential

Osmosis is the net movement of water from higher water potential in a dilute solution to lower water potential in a concentrated solution through a partially permeable membrane.

DETAILED EXPLANATION

  • Water acts as a solvent in digestion, transport and excretion.
  • Water entering a plant cell makes it turgid; the wall resists expansion and creates turgor pressure.
  • Water loss makes a cell flaccid. Severe loss pulls the membrane from the wall, called plasmolysis.
  • Osmosis supports plants and controls water uptake and loss in organisms.
  • To investigate osmosis, cut equal plant-tissue pieces, record their initial mass or length, place them in different concentrations for the same time, blot them dry and calculate the change. Repeats improve reliability.
FORMULA OR EQUATION
percentage change = (final value − initial value) ÷ initial value × 100
LABELLED STUDY DIAGRAMPlant cells in solutions
1dilute outside → water enters → turgid
2balanced movement → flaccid
3concentrated outside → water leaves → plasmolysed
03

SECTION 03

Active transport

Active transport moves molecules or ions against their concentration gradient through a membrane, using energy released by respiration.

DETAILED EXPLANATION

  • Carrier proteins bind particular particles and move them across the membrane.
  • Root hair cells can absorb mineral ions even when the soil contains a lower ion concentration than the cell.
  • Cells performing much active transport need a strong supply of respiratory energy.
LABELLED STUDY DIAGRAMAgainst the gradient
1low concentration
2carrier protein + energy
3high concentration

QUICK CHAPTER SUMMARY

The ideas to carry forward

  • Diffusion moves particles down gradients.
  • Osmosis moves water through a partially permeable membrane.
  • Water gain makes plant cells turgid.
  • Active transport uses respiratory energy and carrier proteins.